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Addgene

pDisplay-GACh2.0
(Plasmid #106073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay vector
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7242
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPCR activation based ACh sensor (GACh) 2.0
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2049
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-GACh2.0 was a gift from Yulong Li (Addgene plasmid # 106073 ; http://n2t.net/addgene:106073 ; RRID:Addgene_106073)

  • For your References section:

    A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies. Jing M, Zhang P, Wang G, Feng J, Mesik L, Zeng J, Jiang H, Wang S, Looby JC, Guagliardo NA, Langma LW, Lu J, Zuo Y, Talmage DA, Role LW, Barrett PQ, Zhang LI, Luo M, Song Y, Zhu JJ, Li Y. Nat Biotechnol. 2018 Sep;36(8):726-737. doi: 10.1038/nbt.4184. Epub 2018 Jul 9. 10.1038/nbt.4184 PubMed 29985477
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