sgLenti
(Plasmid
#105996)
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Purpose(Empty Backbone) single sgRNA expression vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression, Lentiviral, CRISPR ; S.pyogenes sgRNA expression vector
- Promoter U6
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Selectable markersPuromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is the 'empty' sgRNA expression vector with a 1286 bp stuffer sequence. AarI digest cuts out the stuffer sequence and leaves sticky ends on the backbone. sgRNA inserts should be of the format 5'-TTGG(N20) for the top strand and 5'-AAAC-(N20) for the bottom strand for T4 cloning.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgLenti was a gift from Mike McManus (Addgene plasmid # 105996 ; http://n2t.net/addgene:105996 ; RRID:Addgene_105996) -
For your References section:
Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT. Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062. 10.1038/nbt.4062 PubMed 29334369
