pISH-Trpm5
(Plasmid
#105993)
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PurposeTo prepare riboprobe for in situ hybridization
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105993 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
- Backbone size w/o insert (bp) 3015
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Vector typeto generate riboprobe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrpm5
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SpeciesM. musculus (mouse)
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Insert Size (bp)1049
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Entrez GeneTrpm5 (a.k.a. 9430099A16Rik, LTrpC-5, Ltrpc5, Mtr1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with SacII, use SP6 RNA polymerase for IVT.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Trpm5 was a gift from Peter Mombaerts (Addgene plasmid # 105993 ; http://n2t.net/addgene:105993 ; RRID:Addgene_105993) -
For your References section:
Expression of homeobox genes in the mouse olfactory epithelium. Parrilla M, Chang I, Degl'Innocenti A, Omura M. J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19. 10.1002/cne.24051 PubMed 27243442
