pISH-Th
(Plasmid
#105988)
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PurposeTo prepare riboprobe for in situ hybridization
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T
- Backbone size w/o insert (bp) 3000
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Vector typeto generate riboprobe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametyrosine hydroxylase
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SpeciesM. musculus (mouse)
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Insert Size (bp)1639
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GenBank IDNM_009377
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Entrez GeneTh
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linearize with NcoI, use SP6 RNA polymerase for IVT.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Th was a gift from Peter Mombaerts (Addgene plasmid # 105988 ; http://n2t.net/addgene:105988 ; RRID:Addgene_105988) -
For your References section:
Development and topography of the lateral olfactory tract in the mouse: imaging by genetically encoded and injected fluorescent markers. Walz A, Omura M, Mombaerts P. J Neurobiol. 2006 Jul . 66(8):835-46. 10.1002/neu.20266 PubMed 16673392
