Skip to main content
Addgene

pAID 1.1 N-T2A-Bsr
(Plasmid #105985)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAID1.1-N
  • Backbone size w/o insert (bp) 7438
  • Total vector size (bp) 7577
  • Modifications to backbone
    insertion of T2A-Bsr selection marker instead of 9myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418), Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli growth is slow
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T2A-Bsr
  • Species
    Synthetic
  • Insert Size (bp)
    477
  • GenBank ID
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAID 1.1 N-T2A-Bsr was a gift from Tatsuo Fukagawa (Addgene plasmid # 105985 ; http://n2t.net/addgene:105985 ; RRID:Addgene_105985)

  • For your References section:

    An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9. Nishimura K, Fukagawa T. Chromosome Res. 2017 Oct;25(3-4):253-260. doi: 10.1007/s10577-017-9559-7. Epub 2017 Jun 6. 10.1007/s10577-017-9559-7 [pii] PubMed 28589221
Related items:
Commonly requested with: