pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40
(Plasmid #105982)

• Purpose: This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
• Depositing Lab: Marcel Bruchez
• Publication: Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019.

Backbone

• Vector backbone: pAAV-FLEX
• Backbone size w/o insert (bp): 4894
• Total vector size (bp): 7240
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Plasmid is very unstable screen clones for recombination. Grow at 28oC if possible.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FLEX-FAPdL5-POST-T2A-dTomato.FLEX
• Species: H. sapiens (human), M. musculus (mouse), S. cerevisiae (budding yeast); Thosea asigna virus
• Insert Size (bp): 2346
• Promoter: human synapsin 1
• Tags / Fusion Proteins:
  • FAPdL5-POST (N terminal on insert)
  • T2A-dTomato (C terminal on insert)
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: KpnI partial (not destroyed)
• 5′ sequencing primer: hSynF
• 3′ sequencing primer: WPRErev

Resource Information

• Supplemental Documents:
  • pAAV.hSyn.FLEX.kappa.dL5POSTsyn.T2A.dTomato.WPRE.SV40.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry