pAAV.hSyn.FAPdL5-POST-T2A-dTomato.WPRE.bGH
(Plasmid #105981)

• Purpose: This AAV plasmid constitutively expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
• Depositing Lab: Marcel Bruchez
• Publication: Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019.

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: PENN vector core
• Backbone size w/o insert (bp): 4643
• Total vector size (bp): 6984
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Highly susceptible to recombination, screen clones. Grow at 28oC if possible.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hSyn-Igkappa-myc-FAPdL5-POST-T2A-dTomato-WPRE-bGH
• Species: H. sapiens (human), M. musculus (mouse), B. taurus (bovine), S. cerevisiae (budding yeast); Thosea asigna virus (T2A), Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)
• Insert Size (bp): 2341
• Promoter: human synapsin 1
• Tags / Fusion Proteins:
  • FAPdL5-POST (N terminal on insert)
  • T2A-dTomato (C terminal on insert)
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: hSynF
• 3′ sequencing primer: WPRErev

Resource Information

• Supplemental Documents:
  • pAAV.hSynapsin.kappa.myc.dL5.POSTsyn.T2A.dTomato.WPRE.bGH
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry