pME GFP-PCNA
(Plasmid #105977)

• Purpose: entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA
• Depositing Lab: Caren Norden
• Publication: Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Backbone

• Vector backbone: p_DONR221(P1-P2)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA
• Alt name: ATLD2
• Species: H. sapiens (human)
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • 515_ GW_PCNA-GFP pME (sequenced).ape
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry