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Addgene

pCS2+ Integrin beta 1b-mKate
(Plasmid #105975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrinbeta1b
  • Species
    D. rerio (zebrafish)
  • Tag / Fusion Protein
    • mKate (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that the T407N found in integrin is not of functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Integrin beta 1b-mKate was a gift from Caren Norden (Addgene plasmid # 105975 ; http://n2t.net/addgene:105975 ; RRID:Addgene_105975)

  • For your References section:

    Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis. Sidhaye J, Norden C. Elife. 2017 Apr 4;6. pii: e22689. doi: 10.7554/eLife.22689. 10.7554/eLife.22689 PubMed 28372636
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