pCS2+Paxillin-mKate
(Plasmid
#105974)
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Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGWpCS2 Dest(R1-R3)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePaxillin
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SpeciesD. rerio (zebrafish)
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Tag
/ Fusion Protein
- mKate (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor notes that the V94A and P149S mutations found in Paxillin are not of functional concern.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+Paxillin-mKate was a gift from Caren Norden (Addgene plasmid # 105974 ; http://n2t.net/addgene:105974 ; RRID:Addgene_105974) -
For your References section:
Concerted action of neuroepithelial basal shrinkage and active epithelial migration ensures efficient optic cup morphogenesis. Sidhaye J, Norden C. Elife. 2017 Apr 4;6. pii: e22689. doi: 10.7554/eLife.22689. 10.7554/eLife.22689 PubMed 28372636
