p3E DN Kif5a
(Plasmid
#105973)
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Purpose3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105973 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGW_pDONR_P2r-P3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKif5a
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SpeciesD. rerio (zebrafish)
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Mutationtruncation of aa 416-1033
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Entrez Genekif5aa (a.k.a. kif, kif5a, si:ch211-166e11.4, v, ver, vertigo, vrt, wu:fj61a1, wu:fj61a10)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E DN Kif5a was a gift from Caren Norden (Addgene plasmid # 105973 ; http://n2t.net/addgene:105973 ; RRID:Addgene_105973) -
For your References section:
Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB. Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206. 10.1038/srep21206 PubMed 26879757
