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hsp70: mKate2-DN Kif5a
(Plasmid #105972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GWpTol2Dest(R4-R3)pA
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kif5a
  • Species
    D. rerio (zebrafish)
  • Mutation
    truncation of aa416-1033
  • Entrez Gene
    kif5aa (a.k.a. kif, kif5a, si:ch211-166e11.4, v, ver, vertigo, vrt, wu:fj61a1, wu:fj61a10)
  • Tag / Fusion Protein
    • mKate2 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsp70: mKate2-DN Kif5a was a gift from Caren Norden (Addgene plasmid # 105972 ; http://n2t.net/addgene:105972 ; RRID:Addgene_105972)

  • For your References section:

    Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB. Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206. 10.1038/srep21206 PubMed 26879757
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