pME mKate2-dynactin1-811
(Plasmid
#105971)
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Purposeentry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep_DONR221(P1-P2)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedynactin1-811
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SpeciesH. sapiens (human)
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Mutationcontains only amino acids 1-811
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Entrez GeneDCTN1 (a.k.a. DAP-150, DP-150, P135)
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Tag
/ Fusion Protein
- mKate2 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor notes that the mutations found in dynactin are not of functional concern.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME mKate2-dynactin1-811 was a gift from Caren Norden (Addgene plasmid # 105971 ; http://n2t.net/addgene:105971 ; RRID:Addgene_105971) -
For your References section:
Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, Norden C, Wilsch-Brauninger M, Huttner WB. Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206. 10.1038/srep21206 PubMed 26879757