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Addgene

pME GFP-DCX
(Plasmid #105962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p_DONR221_(P1-P2)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NP_835365.1
  • Species
    H. sapiens (human)
  • Entrez Gene
    DCX (a.k.a. DBCN, DC, LISX, SCLH, XLIS)
  • Tags / Fusion Proteins
    • 6xHis-thrombin cleavage site (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME GFP-DCX was a gift from Caren Norden (Addgene plasmid # 105962 ; http://n2t.net/addgene:105962 ; RRID:Addgene_105962)

  • For your References section:

    Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina. Icha J, Kunath C, Rocha-Martins M, Norden C. J Cell Biol. 2016 Oct 24;215(2):259-275. 10.1083/jcb.201604095 PubMed 27810916
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