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Addgene

HS-aPKC-CAAX-GFP
(Plasmid #105946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+ hsp70
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aPKC
  • Alt name
    PKC zeta
  • Species
    R. norvegicus (rat)
  • GenBank ID
    NM_022507.1
  • Entrez Gene
    Prkcz (a.k.a. 14-3-3-zetaisoform, Pkcz, r14-3-3)
  • Tags / Fusion Proteins
    • CAAX (C terminal on insert)
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HS-aPKC-CAAX-GFP was a gift from Caren Norden (Addgene plasmid # 105946 ; http://n2t.net/addgene:105946 ; RRID:Addgene_105946)

  • For your References section:

    Interkinetic nuclear migration is centrosome independent and ensures apical cell division to maintain tissue integrity. Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C. Dev Cell. 2015 Jan 26;32(2):203-19. doi: 10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15. 10.1016/j.devcel.2014.12.001 PubMed 25600237
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