HS-OS-Plk4-mKate2
(Plasmid
#105944)
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Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+ hsp70
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePlk4
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SpeciesM. musculus (mouse)
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Mutation1002 to 3704 of Plk4/ deletion of S282-S305.
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Entrez GenePlk4 (a.k.a. 1700028H20, AI385771, S, Sak, Stk1, Stk18)
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Tag
/ Fusion Protein
- mKate2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HS-OS-Plk4-mKate2 was a gift from Caren Norden (Addgene plasmid # 105944 ; http://n2t.net/addgene:105944 ; RRID:Addgene_105944) -
For your References section:
Interkinetic nuclear migration is centrosome independent and ensures apical cell division to maintain tissue integrity. Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C. Dev Cell. 2015 Jan 26;32(2):203-19. doi: 10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15. 10.1016/j.devcel.2014.12.001 PubMed 25600237