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Addgene

HS-PCNA-GFP
(Plasmid #105942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCNA
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCNA (a.k.a. ATLD2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HS-PCNA-GFP was a gift from Caren Norden (Addgene plasmid # 105942 ; http://n2t.net/addgene:105942 ; RRID:Addgene_105942)

  • For your References section:

    Interkinetic nuclear migration is centrosome independent and ensures apical cell division to maintain tissue integrity. Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C. Dev Cell. 2015 Jan 26;32(2):203-19. doi: 10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15. 10.1016/j.devcel.2014.12.001 PubMed 25600237
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