pCS2 PCNA GFP
(Plasmid #105937)

• Purpose: in vitro transcription, cell cycle nuclear marker
• Depositing Lab: Caren Norden
• Publication: Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.

Backbone

• Vector backbone: pCS2+
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCNA
• Species: H. sapiens (human)
• Entrez Gene: PCNA (a.k.a. ATLD2)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • 34_pCS2+ PCNA-GFP annotated(3).ape
• A portion of this plasmid was derived from a plasmid made by: PCNA GFP came from M. Cristina Cardoso from the Max Delbrück Center for Molecular Medicine in Berlin. PMID 12504011

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry