pcDNA AXL
(Plasmid #105932)

• Purpose: Mammalian expression of myc-His tagged AXL
• Depositing Lab: Rosa Marina Melillo
• Publication: Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-94. doi: 10.1074/jbc.M112.439422. Epub 2013 Apr 29.

Backbone

• Vector backbone: pcDNA4/TO/myc/HIS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AXL
• Species: H. sapiens (human)
• GenBank ID: AAH32229.1
• Entrez Gene: AXL (a.k.a. ARK, AXL3, JTK11, Tyro7, UFO)
• Promoter: tet-inducible CMV
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry