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Addgene

pEMS1725
(Plasmid #105870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ244

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iCreERT2-FFrt-KanR-FFrt
  • Insert Size (bp)
    3020

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′)
  • 3′ sequencing primer pTR (5'-TGGTAGTGTGGGGACTC-3')
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid commercially synthesized by DNA2.0

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1725 was a gift from Elizabeth Simpson (Addgene plasmid # 105870 ; http://n2t.net/addgene:105870 ; RRID:Addgene_105870)

  • For your References section:

    Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420
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