pEMS1925
(Plasmid
#105869)
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PurposeHigh copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJ344
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiCreERT2
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SpeciesH. sapiens (human)
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Insert Size (bp)3923
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pTF3 (5′-CTCGAAAATAATAAAGGGAAAATCAG-3′)
- 3′ sequencing primer pTR (5'-TGGTAGTGTGGGGACTC-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid commercially synthesized by DNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS1925 was a gift from Elizabeth Simpson (Addgene plasmid # 105869 ; http://n2t.net/addgene:105869 ; RRID:Addgene_105869) -
For your References section:
Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420
Map uploaded by the depositor.
