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PurposeMammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105865 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK-GECO1
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SpeciesSynthetic
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Insert Size (bp)1239
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
bioRxiv--A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein (https://doi.org/10.1101/213082)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-K-GECO1 was a gift from Robert Campbell (Addgene plasmid # 105865 ; http://n2t.net/addgene:105865 ; RRID:Addgene_105865) -
For your References section:
A genetically encoded Ca(2+) indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1186/s12915-018-0480-0. 10.1186/s12915-018-0480-0 PubMed 29338710