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Addgene

pBAD-K-GECO1
(Plasmid #105864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    K-GECO1
  • Species
    Synthetic
  • Insert Size (bp)
    1239
  • Tag / Fusion Protein
    • 6-His Tag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

bioRxiv--A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein (https://doi.org/10.1101/213082)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-K-GECO1 was a gift from Robert Campbell (Addgene plasmid # 105864 ; http://n2t.net/addgene:105864 ; RRID:Addgene_105864)

  • For your References section:

    A genetically encoded Ca(2+) indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1186/s12915-018-0480-0. 10.1186/s12915-018-0480-0 PubMed 29338710
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