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pCMV iGluf
(Plasmid #105833)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV(MinDis) [variant of pDisplay]
  • Backbone manufacturer
    modified from Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6829
  • Modifications to backbone
    From pDisplay: Hemagglutinin (HA) tag removed
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGluSnFR E25D variant
  • Alt name
    iGluf
  • Species
    Escherichia coli (strain K12)
  • Insert Size (bp)
    1569
  • Mutation
    Changed Glu 25 to Asp
  • GenBank ID
    21239023 21239023
  • Promoter CMV
  • Tags / Fusion Proteins
    • PDGFR (C terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCMV(MinDis).iGluSnFR vector was obtained from Addgene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCMV iGluf is the E25D variant of iGluSnFR.
(iGluSnFR was created by Marvin et al.2013)

bioRxiv: https://doi.org/10.1101/233494

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV iGluf was a gift from Katalin Torok (Addgene plasmid # 105833 ; http://n2t.net/addgene:105833 ; RRID:Addgene_105833)

  • For your References section:

    Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert JS, Geeves M, Oertner TG, Torok K. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115. 10.1073/pnas.1720648115 PubMed 29735711
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