pNIC28-Bsa4-ICL1
(Plasmid
#105822)
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PurposeExpression of Mycobacterium tuberculosis icl1 recombinant protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameicl1
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Alt nameisocitrate lyase
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SpeciesMycobacterium tuberculosis
- Promoter T7
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Tag
/ Fusion Protein
- His tag with TEV cleavage (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-Bsa4-ICL1 was a gift from Ivanhoe Leung (Addgene plasmid # 105822 ; http://n2t.net/addgene:105822 ; RRID:Addgene_105822) -
For your References section:
Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors. Bhusal RP, Patel K, Kwai BXC, Swartjes A, Bashiri G, Reynisson J, Sperry J, Leung IKH. Medchemcomm. 2017 Oct 17;8(11):2155-2163. doi: 10.1039/c7md00456g. eCollection 2017 Nov 1. 10.1039/c7md00456g PubMed 30108733
