SP65U1-135a-2
(Plasmid
#105820)
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Purposeplasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSP65
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMIRNA135A2
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SpeciesM. musculus (mouse)
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Entrez GeneMir135a-2 (a.k.a. Mirn135a-2, mir-135a-2)
- Promoter U1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The insert corresponds to MIRNA135A2 precursor
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SP65U1-135a-2 was a gift from Cecilia Mannironi (Addgene plasmid # 105820 ; http://n2t.net/addgene:105820 ; RRID:Addgene_105820)