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Addgene

SP65U1-135a-1
(Plasmid #105819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP65
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIRNA135A1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mir135a-1 (a.k.a. Mir135a01, Mirn135, Mirn135a-1, mir-135a-1)
  • Promoter U1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert corresponds to MIRNA135A1 precursor

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SP65U1-135a-1 was a gift from Cecilia Mannironi (Addgene plasmid # 105819 ; http://n2t.net/addgene:105819 ; RRID:Addgene_105819)

  • For your References section:

    Acute stress alters amygdala microRNA miR-135a and miR-124 expression: inferences for corticosteroid dependent stress response. Mannironi C, Camon J, De Vito F, Biundo A, De Stefano ME, Persiconi I, Bozzoni I, Fragapane P, Mele A, Presutti C. PLoS One. 2013 Sep 4;8(9):e73385. doi: 10.1371/journal.pone.0073385. eCollection 2013. PONE-D-13-02768 [pii] PubMed 24023867
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