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pC0056 - LwCas13a-msfGFP-NES
(Plasmid #105815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti(AMP)
  • Backbone size w/o insert (bp) 9204
  • Total vector size (bp) 13584
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LwCas13a
  • Alt name
    LwaCas13a
  • Alt name
    LwC2c2
  • Alt name
    Cas13a
  • Species
    H. sapiens (human); Leptotrichia wadei
  • Insert Size (bp)
    4380
  • Promoter EF-1a
  • Tags / Fusion Proteins
    • msfGFP-NES-3xHA (C terminal on insert)
    • NES (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0056 - LwCas13a-msfGFP-NES was a gift from Feng Zhang (Addgene plasmid # 105815 ; http://n2t.net/addgene:105815 ; RRID:Addgene_105815)

  • For your References section:

    RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959
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