pD765 HMGCR 3'UTR
(Plasmid
#105766)
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PurposeEncodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST 765
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Backbone manufacturerPromega, psiCheck2
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMGCR 3'UTR
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GeneHMGCR (a.k.a. LDLCQ3, LGMDR28, MYPLG)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3'UTR clones downstream of Renilla luciferase reporter gene
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD765 HMGCR 3'UTR was a gift from Joseph Ziegelbauer (Addgene plasmid # 105766 ; http://n2t.net/addgene:105766 ; RRID:Addgene_105766) -
For your References section:
Viral microRNAs Target a Gene Network, Inhibit STAT Activation, and Suppress Interferon Responses. Ramalingam D, Ziegelbauer JM. Sci Rep. 2017 Jan 19;7:40813. doi: 10.1038/srep40813. 10.1038/srep40813 PubMed 28102325