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Addgene

cp173-mCer3-C1
(Plasmid #105752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cp173-mCer3
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cp173-mCer3-C1 was a gift from Megan Rizzo (Addgene plasmid # 105752 ; http://n2t.net/addgene:105752 ; RRID:Addgene_105752)

  • For your References section:

    Regulation of Glucokinase by Intracellular Calcium Levels in Pancreatic beta Cells. Markwardt ML, Seckinger KM, Rizzo MA. J Biol Chem. 2016 Feb 5;291(6):3000-9. doi: 10.1074/jbc.M115.692160. Epub 2015 Dec 23. 10.1074/jbc.M115.692160 PubMed 26698632
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