pET11a asyn Q79N (TAC-->TAT)
(Plasmid
#105733)
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PurposeBacterial expression of mutant human alpha synuclein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET11a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5677
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha-synuclein Q79N (TAC-->TAT)
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SpeciesH. sapiens (human)
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MutationQ79N
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (TAATACGACTCACTATAGG)
- 3′ sequencing primer T7 term (CCGCTGAGCAATAACTAGC) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET11a asyn Q79N (TAC-->TAT) was a gift from Hilal Lashuel (Addgene plasmid # 105733 ; http://n2t.net/addgene:105733 ; RRID:Addgene_105733)