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Addgene

pT7-7 asyn A53T
(Plasmid #105727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-7
  • Backbone size w/o insert (bp) 2438
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha-synuclein A53T
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    476
  • Mutation
    A53T
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 (TAATACGACTCACTATAGG)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-7 asyn A53T was a gift from Hilal Lashuel (Addgene plasmid # 105727 ; http://n2t.net/addgene:105727 ; RRID:Addgene_105727)

  • For your References section:

    E46K Parkinson's-linked mutation enhances C-terminal-to-N-terminal contacts in alpha-synuclein. Rospigliosi CC, McClendon S, Schmid AW, Ramlall TF, Barre P, Lashuel HA, Eliezer D. J Mol Biol. 2009 May 22;388(5):1022-32. doi: 10.1016/j.jmb.2009.03.065. Epub 2009 Apr 5. 10.1016/j.jmb.2009.03.065 PubMed 19345692
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