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Addgene

AAV-GFAP-mNeonGreen
(Plasmid #105713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Original vector is from ADDGENE plasmid #50473
  • Backbone size w/o insert (bp) 711
  • Total vector size (bp) 5503
  • Modifications to backbone
    Replaced GFP with mNeonGreen
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Synthetic; Branchiostoma lanceolatum
  • Insert Size (bp)
    711
  • GenBank ID
    AGG56535
  • Promoter Minimal GFAP promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GFAP-mNeonGreen was a gift from Pantelis Tsoulfas (Addgene plasmid # 105713 ; http://n2t.net/addgene:105713 ; RRID:Addgene_105713)
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