MT4_C30Y R31W
(Plasmid #105712)

• Purpose: Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution
• Depositing Lab: Artur Krężel
• Publication: Krezel Lab Plasmids (unpublished)

Backbone

• Vector backbone: pTyb21
• Backbone manufacturer: NEB
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Metallothionein 4
• Species: H. sapiens (human)
• Insert Size (bp): 186
• Mutation: This subisoform has a C30Y and R31W substitution
• Entrez Gene: MT4 (a.k.a. MT-4, MT-IV, MTIV)
• Tag / Fusion Protein:
  • Chiting Binding Domanin-Intein (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SapI (destroyed during cloning)
• 3′ cloning site: PstI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry