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Addgene

MT3
(Plasmid #105710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTyb21
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Metallothionein 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    204
  • Entrez Gene
    MT3 (a.k.a. GIF, GIFB, GRIF, ZnMT3)
  • Tag / Fusion Protein
    • Chiting Binding Domanin-Intein (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SapI (destroyed during cloning)
  • 3′ cloning site PstI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MT3 was a gift from Artur Krężel (Addgene plasmid # 105710 ; http://n2t.net/addgene:105710 ; RRID:Addgene_105710)

  • For your References section:

    Mass Spectrometry-Based Structural Analysis of Cysteine-Rich Metal-Binding Sites in Proteins with MetaOdysseus R Software. Peris-Diaz MD, Guran R, Zitka O, Adam V, Krezel A. J Proteome Res. 2021 Jan 1;20(1):776-785. doi: 10.1021/acs.jproteome.0c00651. Epub 2020 Sep 28. 10.1021/acs.jproteome.0c00651 PubMed 32924499
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