MT1G_isoform2
(Plasmid
#105705)
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PurposeBacterial expression plasmids for production of recombinant metallothionein 1g subisoform 2 with deletion of Alanine 10
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTyb21
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMetallothionein 1g
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SpeciesH. sapiens (human)
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Insert Size (bp)183
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MutationThis subisoform has a deletion of alanine 10
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Entrez GeneMT1G (a.k.a. MT1, MT1K)
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Tag
/ Fusion Protein
- Chiting Binding Domanin-Intein (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SapI (destroyed during cloning)
- 3′ cloning site PstI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MT1G_isoform2 was a gift from Artur Krężel (Addgene plasmid # 105705 ; http://n2t.net/addgene:105705 ; RRID:Addgene_105705)