MT2a_K20A
(Plasmid
#105698)
-
PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 105698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTyb21
-
Backbone manufacturerNEB
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMetallothionein 2a
-
SpeciesH. sapiens (human)
-
Insert Size (bp)183
-
MutationLysine 20 to Alanine
-
Entrez GeneMT2A (a.k.a. MT-2, MT-II, MT2)
-
Tag
/ Fusion Protein
- Chiting Binding Domanin-Intein (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SapI (destroyed during cloning)
- 3′ cloning site PstI (not destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MT2a_K20A was a gift from Artur Krężel (Addgene plasmid # 105698 ; http://n2t.net/addgene:105698 ; RRID:Addgene_105698) -
For your References section:
The connection of alpha- and beta-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties. Singh AK, Pomorski A, Wu S, Peris-Diaz MD, Czepczynska-Krezel H, Krezel A. Metallomics. 2023 Jun 1;15(6):mfad029. doi: 10.1093/mtomcs/mfad029. 10.1093/mtomcs/mfad029 PubMed 37147085