pcDNA3.1-ZNF598-TEV-3xFLAG
(Plasmid #105690)

• Purpose: Expresses C terminally 3xFLAG tagged ZNF598 protein
• Depositing Lab: Ramanujan Hegde
• Publication: Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4. doi: 10.1016/j.molcel.2016.11.039. Epub 2017 Jan 5.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5059
• Total vector size (bp): 7768
• Modifications to backbone: TEV-3xFLAG was inserted into the vector to serve as a C-terminal tag
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZNF598
• Alt name: zinc finger protein 598
• Species: H. sapiens (human)
• Insert Size (bp): 2717
• GenBank ID: NM_178167
• Entrez Gene: ZNF598 (a.k.a. HEL2)
• Promoter: CMV (from vector)
• Tag / Fusion Protein:
  • TEV-3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: GGTCTATATAAGCAGAGC
• 3′ sequencing primer: GCCTGCTATTGTCTTCCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ZNF598 CDA was originally cloned from the origene plasmid RC208429.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry