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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
(Plasmid #105680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4968
  • Total vector size (bp) 5733
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT1-NTerm: Exon1-Exon2-Exon3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    765
  • Mutation
    Synonymous base changes not affecting the protein sequence
  • Entrez Gene
    Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
  • Promoter tac_Promoter
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer PGEX_5'
  • 3′ sequencing primer PGEX_3_Primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3 was a gift from Ullas Kolthur-Seetharam (Addgene plasmid # 105680 ; http://n2t.net/addgene:105680 ; RRID:Addgene_105680)

  • For your References section:

    Identification of a Tissue-Restricted Isoform of SIRT1 Defines a Regulatory Domain that Encodes Specificity. Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, Kolthur-Seetharam U. Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 10.1016/j.celrep.2017.03.012. 10.1016/j.celrep.2017.03.012 PubMed 28355560
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