pCDZ2FE_Rev3_1873-2025_4PA
(Plasmid
#105673)
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PurposeExpresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3zeo Flag
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRev3L
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SpeciesH. sapiens (human)
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Insert Size (bp)456
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MutationMutations: P1880A, P1885A, P1996A, P2001A
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Entrez GeneREV3L (a.k.a. POLZ, REV3)
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Tag
/ Fusion Protein
- 2X Flag tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CGTGTACGGTGGGAGGTC
- 3′ sequencing primer AGGAAAGGACAGTGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDZ2FE_Rev3_1873-2025_4PA was a gift from Thanos Halazonetis (Addgene plasmid # 105673 ; http://n2t.net/addgene:105673 ; RRID:Addgene_105673)
