Tol2-BetaActin-SecA5-YFP
(Plasmid #105664)

• Purpose: Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter
• Depositing Lab: Qing Deng
• Publication: Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 10.3389/fimmu.2018.00607. eCollection 2018.

Backbone

• Vector backbone: Tol2-pDest
• Backbone manufacturer: Gateway
• Backbone size w/o insert (bp): 11600
• Total vector size (bp): 13378
• Modifications to backbone: Beta-Actin 5' Promoter, PolyA 3'
• Vector type: Tol2-Gateway

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Secreted Annexin5-EYFP
• Alt name: ANXA5
• Species: H. sapiens (human)
• Insert Size (bp): 1784
• Entrez Gene: ANXA5 (a.k.a. ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph)
• Promoter: Beta-Actin
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: TATAGGGCGAATTGGGTACCGCCACCATGCATAAGGTTT
• 3′ sequencing primer: ACCGCGGTGGCGGCCGCTTACTTGTACAGCTCGTCC

Resource Information

• Supplemental Documents:
  • Beta-Actin - SecA-YFP.txt.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry