pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
(Plasmid #105648)

• Purpose: Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
• Depositing Lab: Sandra Rieger
• Publication: Rieger lab plasmids (unpublished)

Backbone

• Vector backbone: pDESTTol2pA2
• Backbone manufacturer: Chi-Bin Chien lab
• Backbone size w/o insert (bp): 5800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Tol2-Isl2b:GFP:polyA
• Alt name: isl3
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2000
• Entrez Gene: isl2b (a.k.a. ISL-3, isl3, islet 3, zfIsl-3, zgc:109840)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from two plasmids from Chi-Bin Chien’s lab at the University of Utah.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results found additional amino acids GGGRSGNSRPLEPLDSAAL at the C-terminus of the EGFP translation. Plasmid was validated and confirmed to function as intended by the depositing laboratory