pT2KXIG (Tol2)-tp63:ikk1C179A:GFP
(Plasmid #105647)

• Purpose: Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes
• Depositing Lab: Sandra Rieger
• Publication: Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub 2017 Jan 25.

Backbone

• Vector backbone: pT2KXIG (Tol2)
• Backbone manufacturer: Kawakami lab, National Genome Institute Japan
• Backbone size w/o insert (bp): 9768
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tp63:ikk1C179A:GFP
• Alt name: IKK alpha
• Alt name: chuk
• Species: D. rerio (zebrafish)
• Insert Size (bp): 9500
• Mutation: p63 promoter + ikk1C179A + GFP
• Entrez Gene: chuk (a.k.a. MGC56539, ikk1, zgc:56539)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry