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pT2KXIG (Tol2)-tp63:ikk1KD:GFP
(Plasmid #105645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT2KXIG (Tol2)
  • Backbone manufacturer
    Kawakami lab, National Genome Institute Japan
  • Backbone size w/o insert (bp) 9768
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tp63:ikk1KD:GFP
  • Alt name
    IKK alpha
  • Alt name
    chuk
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    9500
  • Mutation
    p63 promoter + ikk1 (kinase domain deleted) + GFP
  • Entrez Gene
    chuk (a.k.a. MGC56539, ikk1, zgc:56539)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2KXIG (Tol2)-tp63:ikk1KD:GFP was a gift from Sandra Rieger (Addgene plasmid # 105645 ; http://n2t.net/addgene:105645 ; RRID:Addgene_105645)

  • For your References section:

    IKKalpha regulates human keratinocyte migration through surveillance of the redox environment. Lisse TS, Rieger S. J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub 2017 Jan 25. 10.1242/jcs.197343 PubMed 28122935
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