ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato
(Plasmid #105644)

• Purpose: Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine
• Depositing Lab: Sandra Rieger
• Publication: Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub 2017 Jan 25.

Backbone

• Vector backbone: ptdTomato-N1 (Clontech)
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5443
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CMVL-ikk1FL(C179A)-tdTomato
• Alt name: IKK alpha
• Alt name: chuk
• Species: D. rerio (zebrafish)
• Insert Size (bp): 6000
• Mutation: CMV:ikk1FL(C178A)-tdTomato
• Entrez Gene: chuk (a.k.a. MGC56539, ikk1, zgc:56539)
• Tag / Fusion Protein:
  • tdTomato (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry