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Addgene

pIRESN2_GFP_YTA_Champ1_328-354
(Plasmid #105639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo2 GFP YTA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Champ1
  • Alt name
    CAMP, C13orf8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    78
  • Entrez Gene
    CHAMP1 (a.k.a. C13orf8, CAMP, CHAMP, MRD40, ZNF828)
  • Tag / Fusion Protein
    • GFP YTA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CGTGTACGGTGGGAGGTC
  • 3′ sequencing primer GCCTGCCACACCTCAAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESN2_GFP_YTA_Champ1_328-354 was a gift from Thanos Halazonetis (Addgene plasmid # 105639 ; http://n2t.net/addgene:105639 ; RRID:Addgene_105639)
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