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pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014
(Plasmid #105637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT5T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Rev7
  • Alt name
    Mad2B, Mad2l2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    633
  • Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
  • Entrez Gene
    Mad2l2 (a.k.a. 2310033C13Rik, G1-453-4, MAD2B, REV7, repro22)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SmaI (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAG
  • 3′ sequencing primer CAA GAC CCG TTT AGA GGC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Rev3l
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    75
  • Entrez Gene
    REV3L (a.k.a. POLZ, REV3)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAG
  • 3′ sequencing primer CAA GAC CCG TTT AGA GGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014 was a gift from Thanos Halazonetis (Addgene plasmid # 105637 ; http://n2t.net/addgene:105637 ; RRID:Addgene_105637)
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