pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898
(Plasmid
#105635)
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PurposeExpresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT5T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameRev7
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Alt nameMad2B, Mad2l2
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SpeciesM. musculus (mouse)
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Insert Size (bp)633
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MutationMutations: F11S, G12A, V132K, C133V, A135K
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Entrez GeneMad2l2 (a.k.a. 2310033C13Rik, G1-453-4, MAD2B, REV7, repro22)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer TAATACGACTCACTATAG
- 3′ sequencing primer CAA GAC CCG TTT AGA GGC (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameRev3l
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SpeciesH. sapiens (human)
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Insert Size (bp)75
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Entrez GeneREV3L (a.k.a. POLZ, REV3)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer TAATACGACTCACTATAG
- 3′ sequencing primer CAA GAC CCG TTT AGA GGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898 was a gift from Thanos Halazonetis (Addgene plasmid # 105635 ; http://n2t.net/addgene:105635 ; RRID:Addgene_105635)