pET30b-7D-SrtA
(Plasmid
#105600)
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Purposeexpresses 7D sortase A variant in E.coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 105600 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET30b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS.aureus Sortase A, 7D mutant
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SpeciesS.aureus
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Insert Size (bp)500
- Promoter T7
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Tag
/ Fusion Protein
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30b-7D-SrtA was a gift from Stephanie Dougan (Addgene plasmid # 105600 ; http://n2t.net/addgene:105600 ; RRID:Addgene_105600) -
For your References section:
Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling. Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK. PLoS One. 2017 Dec 4;12(12):e0189068. doi: 10.1371/journal.pone.0189068. eCollection 2017. PONE-D-17-29293 [pii] PubMed 29200433
