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Addgene

pAAV.GfaABC1D.PI.Lck-GFP.SV40
(Plasmid #105598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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AAV5 105598-AAV5 Virus (100 µL at titer ≥ 7×10¹² vg/mL) and Plasmid. $405
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Backbone

  • Vector backbone
    pZac2.1
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lck-EGFP
  • Promoter GfaABC1D

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Penn Vector Core plasmid p2369

Information for AAV5 (Catalog # 105598-AAV5) ( Back to top)

Purpose

Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA.

GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.

Delivery

  • Volume 100 µL
  • Titer ≥ 7×10¹² vg/mL
  • Pricing $375 USD for preparation of 100 µL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
  • Buffer PBS + 0.001% Poloxamer 188
  • Serotype AAV5
  • Purification Iodixanol gradient ultracentrifugation
  • Reporter Gene GFP

Biosafety

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Quality Control:
  • Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
  • Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.GfaABC1D.PI.Lck-GFP.SV40 was a gift from Baljit Khakh (Addgene plasmid # 105598 ; http://n2t.net/addgene:105598 ; RRID:Addgene_105598) For viral preps, please replace (Addgene plasmid # 105598) in the above sentence with: (Addgene viral prep # 105598-AAV5)

  • For your References section:

    Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses. Shigetomi E, Bushong EA, Haustein MD, Tong X, Jackson-Weaver O, Kracun S, Xu J, Sofroniew MV, Ellisman MH, Khakh BS. J Gen Physiol. 2013 Apr 15. 10.1085/jgp.201210949 PubMed 23589582
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